Developing disease‐resistant thermosensitive male sterile rice by multiplex gene editing
نویسندگان
چکیده
منابع مشابه
Multiplex Gene Editing in Rice Using the CRISPR-Cpf1 System.
The class 2/type II clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 system has been used successfully for simultaneous modification of multiple loci in plants. Two general strategies have been applied to coexpressmultiple single guide RNAs (sgRNAs) to achieve multiplex gene editing in plant cells. One is to construct the multiple guide RNA expression cassettes into separa...
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The utilization of thermosensitive genic male sterility system (TGMS) has great potential for revolutionizing hybrid rice production in tropical countries through simple, less expensive and efficient seed production technology without any limitation in fertility restoration which have been the hindrance for commercial exploitation of heterosis in rice using cytoplasmic genic male sterility syst...
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In most flowering plant species, pollination and fertilization occur during the hot summer, so plants must have evolved a mechanism that ensures normal growth of their pollen tubes at high temperatures. Despite its importance to plant reproduction, little is known about the molecular basis of thermotolerance in pollen tubes. Here we report the identification and characterization of a novel Arab...
متن کاملEngineered Dwarf Male-Sterile Rice: A Promising Genetic Tool for Facilitating Recurrent Selection in Rice
Rice is a crop feeding half of the world's population. With the continuous raise of yield potential via genetic improvement, rice breeding has entered an era where multiple genes conferring complex traits must be efficiently manipulated to increase rice yield further. Recurrent selection is a sound strategy for manipulating multiple genes and it has been successfully performed in allogamous cro...
متن کاملDevelopment of Commercial Thermo-sensitive Genic Male Sterile Rice Accelerates Hybrid Rice Breeding Using the CRISPR/Cas9-mediated TMS5 Editing System
Hybrid rice breeding offers an important strategy to improve rice production, in which the cultivation of a male sterile line is the key to the success of cross-breeding. CRISPR/Cas9 systems have been widely used in target-site genome editing, whereas their application for crop genetic improvement has been rarely reported. Here, using the CRISPR/Cas9 system, we induced specific mutations in TMS...
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ژورنال
عنوان ژورنال: Journal of Integrative Plant Biology
سال: 2019
ISSN: 1672-9072,1744-7909
DOI: 10.1111/jipb.12774